aditec mra 500 manual

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aditec mra 500 manual

MIC 1018 (senkrecht) MIC 1218 (waagerecht) MIC 200F (senkrecht) MIC 100F (waagerecht) Prozess- Steuerung fur Universalanlagen Sie ist in weiten Bereichen frei einstellbar und leicht auf viele Einsatzzwecke anzupassen. Die Steuerung kann 99 Programme mit je 9 Schritten speichern. Es werden 8 (MIC 08) bzw. 8 (MIC 00F) potentialfreie Ausgangsrelais angesteuert. Zur Anpassung an den jeweiligen Einsatzzweck kann jedes Aggregat als -Punkt-Regler oder Xp-Regler eingestellt werden. Programm- und Schrittanzeige zeigen keine Werte. Programm- werden dargestellt. Nach dem Programmablauf wird das Programmende angezeigt und Signal gegeben. Programmierung und Einstellungen: Im werden nur die Sollwerte und Behandlungsarten angezeigt, die Istwertanzeigen sind aus. Die LED der Taste leuchtet. Der dient der Eingabe von Programmen und der Einstellung (Konfiguration) der Steuerung. Im leuchtet die LED der Taste. Die Steuerung geht in den sobald Sie eine eingeben. Sollte SAFE angezeigt werden ist der Programmierschutz aktiv. Fragen sie Ihren Servicetechniker. ACHTUNG: Wenn in einem Programmschritt noch keine Behandlungsart definiert ist, leuchten alle LED s in den Zifferntasten hintereinander auf. In diesem Fall ist die Sollwerteingabe in diesem Schritt gesperrt, bis eine Behandlungsart eingegeben ist. Seite 6. Programmbeispiele. Kammertemperaturregelung Voraussetzung: Beginn des Neue Behandlungsart festlegen Kammertemperatur Die LED der Taste ist an. Kammertemperaturanzeige 6 Laufzeit (Schrittzeit) wird wie eingegeben angezeigt. LED in Taste ist aus Seite 6 7. Deltatemperaturregelung Voraussetzung: Beginn des Neue Behandlungsart festlegen Kammertemperatur 0 6 Die LED der Taste ist an. Der eingegebene Kammersollwert dient als obere Begrenzung der Kammertemperaturregelung. Bei Programmen mit Deltatemperatur darf nicht gleichzeitig eine Fc-Sollwert eingegeben werden. Die Fc-Wert-Bestimmung Der Fc-Wert wird im Abstand von Minute aus der Kern-Ist-Temperatur ermittelt und aufsummiert.

Da sich bei Temperaturen unter Grad ein Fc-Wert von Null ergibt, erfolgt die Aufsummierung erst ab dieser Temperaturschwelle. Fc-Wert-Abschaltung Voraussetzung: Beginn des Neue Behandlungsart festlegen Kammertemperatur Die LED der Taste ist an. FC wird im Feuchte- Ist-Feld angezeigt. Bei Programmen mit Fc-Soll-Wert darf nicht gleichzeitig eine Deltatemperatur eingegeben werden. Beginn des Neue Behandlungsart festlegen Kammertemperatur Die LED der Taste ist an. Kammertemperaturanzeige 6 Kernsolltemperatur wird wie eingegeben angezeigt. Bitte Unterschied zur negativen Kernabschaltung beachten. Bitte Unterschied zur positiven Kernabschaltung beachten. Siehe Serviceanleitung Kapitel Relaisschaltverhalten. Befeuchtungsollwert Seite 13 Eingabe des Befeuchtungsollwerts Der Befeuchtungsollwert wird wie eingegeben angezeigt. Die Einschaltzeit ist im Relaisschaltverhalten festgelegt. Beginn des Die LED der Taste ist an. Entfeuchtungsollwert. Es wird ein Minus davor angezeigt. 7 Eingabe des Entfeuchtungsollwertes 9 6 Der Entfeuchtungsollwert wird wie eingegeben mit einem Minus davor angezeigt. Beginn des Neue Behandlungsart festlegen Kammertemperatur 0 Die LED der Taste ist an. Kammertemperaturanzeige wird wie eingegeben angezeigt. Seite 7 18 Erstellen eines neuen Programmes Neue eingeben. Pgm Nr Beenden des beenden LED in Taste ist aus Seite 8 19. Die Anzeige blinkt Anzeige Notlauf-Feuchte- Sollwert mit kleinem vorangestelltem n. Der Notlauf-Feuchte- Sollwert wird wie angegeben angezeigt. Die Einzeit ist % von 0 Sekunden, ergibt Sekunden. Die Auszeit ist 7% von 0 Sekunden ergibt Sekunden. Beenden des beenden LED in Taste ist aus Seite 9 20. Das Relais kann dann in den Behandlungsarten mit anderem Schaltverhalten verwendet werden (siehe Serviceanleitung unter Kapitel Aggregate bestimmen folgende). Von-Schritt und Bis-Schritt werden in der Anzeige Schrittnummer rotierend angezeigt. Da beide gleich sind ist dies hier nicht zuerkennen.

Von-Schritt und Bis-Schritt werden abwechselnd angezeigt.Seite 6 27.7 Ruhephase zwischen den Schritten Voraussetzung: 6 Beginn des Neue Behandlungsart festlegen Schrittlaufzeit 0 Die LED der Taste ist an. Beenden des beenden LED in Taste ist aus Hinweis: Die Pausezeit von Minuten ist in der Gesamtschrittzeit von 0 Minuten enthalten. Soll ein Schritt nur Pause sein, muss die Pausezeit gleich der Schrittzeit eingegeben werden.8 Programmierung der Behandlungsarten bis 0 in einem Programmschritt. Die LED der Taste ist an. Die Anzeige blinkt Anzeige Die LED in der Zifferntaste Seite 8 29.9 Einzelschrittsteuerung Die Funktion Einzelschrittsteuerung kann auf zwei Arten konfiguriert sein. Die Schrittnummer und die LED in der Taste Prog blinken, d.h. die Einzelschrittsteuerung ist aktiviert. Die Schrittanzeige wird nicht mehr blinkend dargestellt. Die LED in der Taste Prog erlischt. Die Anzeige blinkt Anzeige Programmschritt wird angezeigt. Alle Sollwerte werden mit 000 angezeigt. Seite 0 31 Beenden des beenden LED in Taste ist aus. Der bisherige Schritt wird zu Schritt usw.Die bisherigen Schritte bis 6 wurden zu Schritt bis 7. Schritt kann programmiert werden. Seite 32 7 Beenden des beenden LED in Taste ist aus.Die bisherigen Schritte bis 7 wurden zu Schritt bis 6. 6 Beenden des beenden LED in Taste ist aus Seite 33. Der aktueller Fc-Wert wird angezeigt.Eingeben des datums. Das eingegebene datum wird blinkend angezeigt 0 6 en Programm mit neuer zeit und datum. Eine leuchtende LED bedeutet im Betriebszustand, das Relais (Aggregate) ist eingeschaltet. Aktuelle Uhrzeit Der Dezimalpunkt in der Anzeige springt im Sekundenrhythmus zwischen der zweiten und der vierten Anzeigestelle. Die Anzeige blinkt dann.Die Anzeige blinkt dann.Einstellung der Funktionen Regelgerat KR Bitte vor Bedienung sorgfaltig lesen Digitale T-Bar T-4 Elektronikschloss E Paderborn Pamplonastra?e 2 Die Spannungsversorgung der Regelung Die Schaltuhr kann als Tages- bzw. Wochenschaltuhr eingesetzt werden. 1.

1 Folgene Funktionen zeichnen die Uhr aus: Als Startintervall konnen 60, 30 oder 10 Sekunden ausgewahlt Blockstruktur. Seite 1 von 5 Die Bedienung uber die Folientastatur Inhaltsverzeichnis. Inhaltsverzeichnis.2 2. Sicherheitshinweis.2 Schwere Verletzungen, Brand oder Sachschaden moglich. Nachdruck, auch auszugsweise, nur mit unserer Genehmigung. Anderungen vorbehalten. 2 06.2007 TR30G009 Verbrennungsgefahr, Gerateschaden und Fehlfunktionen. Bei der Installation sind die Richtlinien der VDE 0100 und VDE Die Modelle Hierbei sind die entsprechenden nationalen und lokalen Vorschriften zu beachten. Cody Filtersteuerung 230V. Art.Nr Funktion: Infrarot exclusiv. Infrarot-Kabinensteuerung 230V. Art.Nr Funktion: Oktober 2003 Benutzerinformation n Die Funktionen Schaltaktor Lesen Sie dieses Abweichend gelten folgende technische Daten: 3.0 LCD Anzeige-Einheit Display-Gesamtinhalt: a) aktuelle Uhrzeit Programmierbar am PC mit Software. Seite 1 von 7 TWIN-CENTER-Neueinsteigern To use this website, you must agree to our Privacy Policy, including cookie policy. The detection and characterization of these elements has been mainly limited to plants. Here we present the first finding of a TRIM element in bivalves, and among the first known in the kingdom Animalia. Class Bivalvia has high ecological and commercial importance in marine ecosystems and aquaculture, and, in recent years, an increasing number of genomic studies has addressed to these organisms. We have identified biv-TRIM in several bivalve species: Donax trunculus, Ruditapes decussatus, R. philippinarum, Venerupis corrugata, Polititapes rhomboides, Venus verrucosa, Dosinia exoleta, Glycymeris glycymeris, Cerastoderma edule, Magallana gigas, Mytilus galloprovincialis.In addition to canonically structured elements, solo-TDRs and tandem repeats were detected.

The presence of this element in the genome of each species is The discovery of rare, heterogeneous self-renewing stem cells with shared developmental and molecular features within epithelial components of mammary gland and breast cancers has provided a conceptual framework to understand cellular composition of these tissues and mechanisms that control their number. Several single markers or combinations of markers have been reported to prospectively enrich MaSCs and BCSCs. Such markers and the extent to which they enrich for MaSCs and BCSCs activity require a critical appraisal. Also, knowledge of the functional assays and their limitations and harmonious reporting of results is a prerequisite to improve our understanding of MaSCs and BCSCs. This chapter describes evolution of the concept of MaSCs and BCSCs, and specific methodologies to investigate them. The mycobacterial type VII secretion system ESX-1 is responsible for the secretion of a number of proteins that play important roles during host infection. The regulation of the expression of secreted proteins is often essential to establish successful infection. Using transcriptome sequencing, we found that the abrogation of ESX-1 function in Mycobacterium marinum leads to a pronounced increase in gene expression levels of the espA operon during the infection of macrophages. In addition, the disruption of ESX-1-mediated protein secretion also leads to a specific down-regulation of the ESX-1 substrates, but not of the structural components of this system, during growth in culture medium. This effect is observed in both M. marinum and M. tuberculosis. We established that down-regulation of ESX-1 substrates is the result of a regulatory process that is influenced by the putative transcriptional regulator whib6, which is located adjacent to the esx-1 locus.

Taken together, these data show that WhiB6 is required for the secretion-dependent regulation of ESX-1 substrates and that ESX-1 substrates are regulated independently from the structural components, both during infection and as a result of active secretion. The metaGRS hazard ratio for IS (1.26, 95% CI 1.22-1.31 per metaGRS standard deviation) doubles that of a previous GRS, identifying a subset of individuals at monogenic levels of risk: the top 0.25% of metaGRS have three-fold risk of IS. The metaGRS is similarly or more predictive compared to several risk factors, such as family history, blood pressure, body mass index, and smoking. We estimate the reductions needed in modifiable risk factors for individuals with different levels of genomic risk and suggest that, for individuals with high metaGRS, achieving risk factor levels recommended by current guidelines may be insufficient to mitigate risk. However, are there other species that can also be relevant models. Similarities to humans in terms of environmental exposures, life-span, genetics, histopathology and available therapeutics are all factors that can be considered when looking at species other than the laboratory mouse. This review will explore the occurrence of metastasis in multiple species from a variety of domestic, captive and free-living veterinary cases to assist in identifying potential alternative experimental and clinical research models relevant to humans. The standard workhorse for genomic analysis of the evolution of bacterial populations is phylogenetic modelling of mutations in the core genome. However, a notable amount of information about evolutionary and transmission processes in diverse populations can be lost unless the accessory genome is also taken into consideration.

Here, we introduce panini (Pangenome Neighbour Identification for Bacterial Populations), a computationally scalable method for identifying the neighbours for each isolate in a data set using unsupervised machine learning with stochastic neighbour embedding based on the t-SNE (t-distributed stochastic neighbour embedding) algorithm.Several case studies with single- and multi-clone pneumococcal populations are presented to demonstrate the ability to identify biologically important signals from gene content data.Genome analysis of diverse human populations has contributed to the identification of novel genomic loci for diseases of major clinical and public health impact. Here, we report a genome-wide analysis of type 2 diabetes (T2D) in sub-Saharan Africans, an understudied ancestral group. We also show transferability in our study of 32 established T2D loci. Our findings advance understanding of the genetics of T2D in non-European ancestry populations. Objectives: Systemic sclerosis (SSc) is an autoimmune disease with unknown pathogenesis manifested by inflammation, vasculopathy and fibrosis in skin and internal organs. Type I interferon signature found in SSc propelled us to study plasmacytoid dendritic cells (pDCs) in this disease. We aimed to identify candidate pathways underlying pDC aberrancies in SSc and to validate its function on pDC biology. Methods: In total, 1193 patients with SSc were compared with 1387 healthy donors and 8 patients with localised scleroderma. PCR-based transcription factor profiling and methylation status analyses, single nucleotide polymorphism genotyping by sequencing and flow cytometry analysis were performed in pDCs isolated from the circulation of healthy controls or patients with SSc.Results: Here, we show downregulation of transcription factor RUNX3 in SSc pDCs. A higher methylation status of the RUNX3 gene, which is associated with polymorphism rs6672420, correlates with lower RUNX3 expression and SSc susceptibility.

Hypoxia is another factor that decreases RUNX3 level in pDC. Mouse pDCs deficient of Runx3 show enhanced maturation markers on CpG stimulation. In vivo, deletion of Runx3 in dendritic cell leads to spontaneous induction of skin fibrosis in untreated mice and increased severity of bleomycin-induced skin fibrosis. Conclusions: We show at least two pathways potentially causing low RUNX3 level in SSc pDCs, and we demonstrate the detrimental effect of loss of Runx3 in SSc model further underscoring the role of pDCs in this disease. RSV epidemiological data alone has been insufficient in defining who acquires infection from whom (WAIFW) within households. We investigated RSV genomic variation within and between infected individuals and assessed its potential utility in tracking transmission in households. Over an entire single RSV season in coastal Kenya, nasal swabs were collected from members of 20 households every 3-4 days regardless of symptom status and screened for RSV nucleic acid. Single nucleotide polymorphisms (SNPs) observed during household infection outbreaks ranged from 0-21 (median: 3) while SNPs observed during single-host infection episodes ranged from 0-17 (median: 1). Using the viral genomic data alone there was insufficient resolution to fully reconstruct within-household transmission chains. For households with clear index cases, the most likely source of infant infection was via a toddler (aged 1 to Fat storage-inducing transmembrane protein 2 (FIT2) aids in partitioning of cellular triacylglycerol into lipid droplets. A genome-wide association study reported FITM2-R3H domain containing like-HNF4A locus to be associated with type 2 diabetes (T2DM) in East Asian populations. Mice with adipose tissue (AT)-specific FIT2 knockout exhibited lipodystrophic features, with reduced AT mass, insulin resistance, and greater inflammation in AT when fed a high-fat diet. The role of FIT2 in regulating human adipocyte function is not known.

Here, we found FIT2 protein abundance is lower in subcutaneous and omental AT obtained from patients with T2DM compared with nondiabetic control subjects. Partial loss of FIT2 protein in primary human adipocytes attenuated their lipid storage capacity and induced insulin resistance. After palmitate treatment, triacylglycerol accumulation, insulin-induced Akt (Ser-473) phosphorylation, and insulin-stimulated glucose uptake were significantly reduced in FIT2 knockdown adipocytes compared with control cells.In Seq-Well, uniquely barcoded mRNA capture beads and cells are co-confined in picowells that are sealed using a semipermeable membrane, enabling efficient cell lysis and mRNA capture. The beads are subsequently removed and processed in parallel for sequencing, with each transcript's cell of origin determined via the unique barcodes. Due to its simplicity and portability, Seq-Well can be performed almost anywhere. Here we aim to use the breadth of phenotypic information recorded in DDD to augment diagnosis and disease variant discovery in probands. Pairwise comparisons of individuals with high-impact inherited variants to the 32 individuals with causative DNM in ANKRD11 using only growth z-scores highlighted 5 likely causative inherited variants and two unrecognized DNM resulting in an 18% diagnostic uplift for this gene. Using an independent approach, naive Bayes classification of growth and developmental data produced reasonably discriminative models for the 24 DNM genes with sufficiently complete data. This highlighted heterozygous de novo nonsynonymous variants in SPTBN2 as causative in three DDD probands. Global Mycobacterium tuberculosis population comprises 7 major lineages. The Beijing strains, particularly the ones classified as Modern groups, have been found worldwide, frequently associated with drug resistance, younger ages, outbreaks and appear to be expanding. Here, we report analysis of whole genome sequences of 1170 M.

tuberculosis isolates together with their patient profiles. Our samples belonged to Lineage 1-4 (L1-L4) with those of L1 and L2 being equally dominant. Phylogenetic analysis revealed several new or rare sublineages. Differential associations between sublineages of M. tuberculosis and patient profiles, including ages, ethnicity, HIV (human immunodeficiency virus) infection and drug resistance were demonstrated. The Ancestral Beijing strains and some sublineages of L4 were associated with ethnic minorities while L1 was more common in Thais. L2.2.1.Ancestral 4 surprisingly had a mutation that is typical of the Modern Beijing sublineages and was common in Akha and Lahu tribes who have migrated from Southern China in the last century. This may indicate that the evolutionary transition from the Ancestral to Modern Beijing sublineages might be gradual and occur in Southern China, where the presence of multiple ethnic groups might have allowed for the circulations of various co-evolving sublineages which ultimately lead to the emergence of the Modern Beijing strains. Background: Functional characterisation of the compact genome of the model organism Caenorhabditis elegans remains incomplete despite its sequencing 20 years ago. The last decade of research has seen a tremendous increase in the number of non-coding RNAs identified in various organisms. While we have mechanistic understandings of small non-coding RNA pathways, long non-coding RNAs represent a diverse class of active transcripts whose function remains less well characterised. Results: By analysing hundreds of published transcriptome datasets, we annotated 3392 potential lncRNAs including 143 multi-exonic loci that showed increased nucleotide conservation and GC content relative to other non-coding regions. Using automated microscopy for in-depth phenotyping, we show that six of the long non-coding RNA loci are required for normal development and fertility.

Using RNA interference-mediated gene knock-down, we provide evidence that for two of the long non-coding RNA loci, the observed phenotypes are dependent on the corresponding RNA transcripts. Conclusions: Our results highlight that a large section of the non-coding regions of the C. elegans genome remains unexplored. Based on our in vivo analysis of a selection of high-confidence lncRNA loci, we expect that a significant proportion of these high-confidence regions is likely to have a biological function at either the genomic or the transcript level. The Plasmodium falciparum reticulocyte-binding protein homolog 5 (PfRH5) is the leading target for next-generation vaccines against the disease-causing blood-stage of malaria. However, little is known about how human antibodies confer functional immunity against this antigen. We isolated a panel of human monoclonal antibodies (mAbs) against PfRH5 from peripheral blood B cells from vaccinees in the first clinical trial of a PfRH5-based vaccine. We identified a subset of mAbs with neutralizing activity that bind to three distinct sites and another subset of mAbs that are non-functional, or even antagonistic to neutralizing antibodies. We also identify the epitope of a novel group of non-neutralizing antibodies that significantly reduce the speed of red blood cell invasion by the merozoite, thereby potentiating the effect of all neutralizing PfRH5 antibodies as well as synergizing with antibodies targeting other malaria invasion proteins. Our results provide a roadmap for structure-guided vaccine development to maximize antibody efficacy against blood-stage malaria. Genetic variants regulating RNA splicing and transcript usage have been implicated in both common and rare diseases.

Although transcript usage quantitative trait loci (tuQTLs) have been mapped across multiple cell types and contexts, it is challenging to distinguish between the main molecular mechanisms controlling transcript usage: promoter choice, splicing and 3' end choice. Here, we analysed RNA-seq data from human macrophages exposed to three inflammatory and one metabolic stimulus. In addition to conventional gene-level and transcript-level analyses, we also directly quantified promoter usage, splicing and 3' end usage. We found that promoters, splicing and 3' ends were predominantly controlled by independent genetic variants enriched in distinct genomic features. Promoter usage QTLs were also 50% more likely to be context-specific than other tuQTLs and constituted 25% of the transcript-level colocalisations with complex traits. Thus, promoter usage might be an underappreciated molecular mechanism mediating complex trait associations in a context-specific manner. Background: Salmonella enterica serovar Enteritidis is a cause of both poultry- and egg-associated enterocolitis globally and bloodstream-invasive nontyphoidal Salmonella (iNTS) disease in sub-Saharan Africa (sSA). Distinct, multi-drug resistant genotypes associated with iNTS disease in sSA have recently been described, often requiring treatment with fluoroquinolone antibiotics. In industrialised countries, antimicrobial use in poultry production has led to frequent fluoroquinolone resistance amongst globally prevalent enterocolitis-associated lineages. These isolates, notable for a high rate of diminished ciprofloxacin susceptibility (DCS), were placed in the phyletic context of 1,067 sequences from the Public Health England (PHE) S. Enteritidis genome database to understand whether DCS was associated with African or globally-circulating clades of S. Enteritidis. Analysis showed four of the major S. Enteritidis clades were represented, two global and two African.

All thirteen DCS isolates, containing a single gyrA mutation at codon 87, belonged to a global PT4-like clade responsible for epidemics of poultry-associated enterocolitis. Apart from two DCS isolates, which clustered with PHE isolates associated with travel to Spain and Brazil, the remaining DCS isolates, including one poultry isolate, belonged to two monophyletic clusters in which gyrA 87 mutations appear to have developed within the region. Antimicrobial resistance profiles differed by clade, highlighting the challenges of devising empirical sepsis guidelines. The detection of fluoroquinolone resistance in phyletically-related poultry and human isolates is of major concern and surveillance and control measures within the region's burgeoning poultry industry are required to protect a human population at high risk of iNTS disease. Introduction: Health insurance has been found to increase healthcare utilisation and reduce catastrophic health expenditures in a number of countries; however, coverage is often unequally distributed among populations. The sociodemographic patterns of health insurance in Namibia are not fully understood. We aimed to assess the prevalence of health insurance, the relation between health insurance and health service utilisation and to explore the sociodemographic factors associated with health insurance in Namibia. Such findings may help to inform health policy to improve financial access to healthcare in the country. Methods: Using data on 14,443 individuals, aged 15 to 64 years, from the 2013 Namibia Demographic and Health Survey, the association between health insurance and health service utilisation was investigated using multivariable mixed effects Poisson regression analyses, adjusted for sociodemographic covariates and regional, enumeration area and household clustering.

Multivariable mixed effects Poisson regression analyses were also conducted to explore the association between key sociodemographic factors and health insurance, adjusted for covariates and clustering. Effect modification by sex, education level and wealth quintile was also explored. Education may play a key role in health insurance coverage, especially for women and the less wealthy. These findings may help to inform the targeting of strategies to improve financial protection from healthcare-associated costs in Namibia. However, their range of applications remains limited by signal variability from different guide RNAs that target the same gene, which confounds gene effect estimation and dictates large experiment sizes. To address this problem, we report JACKS, a Bayesian method that jointly analyzes screens performed with the same guide RNA library. Modeling the variable guide efficacies greatly improves hit identification over processing a single screen at a time and outperforms existing methods. This more efficient analysis gives additional hits and allows designing libraries with a 2.5-fold reduction in required cell numbers without sacrificing performance compared to current analysis standards. Despite extensive culturing and sequencing efforts, the complete bacterial repertoire of the human gut microbiota remains undefined. Here we identify 1,952 uncultured candidate bacterial species by reconstructing 92,143 metagenome-assembled genomes from 11,850 human gut microbiomes. These uncultured genomes substantially expand the known species repertoire of the collective human gut microbiota, with a 281% increase in phylogenetic diversity. Although the newly identified species are less prevalent in well-studied populations compared to reference isolate genomes, they improve classification of understudied African and South American samples by more than 200%.

These candidate species encode hundreds of newly identified biosynthetic gene clusters and possess a distinctive functional capacity that might explain their elusive nature. Our work expands the known diversity of uncultured gut bacteria, which provides unprecedented resolution for taxonomic and functional characterization of the intestinal microbiota. Following severe or chronic liver injury, adult ductal cells (cholangiocytes) contribute to regeneration by restoring both hepatocytes and cholangiocytes. We recently showed that ductal cells clonally expand as self-renewing liver organoids that retain their differentiation capacity into both hepatocytes and ductal cells. However, the molecular mechanisms by which adult ductal-committed cells acquire cellular plasticity, initiate organoids and regenerate the damaged tissue remain largely unknown. Here, we describe that ductal cells undergo a transient, genome-wide, remodelling of their transcriptome and epigenome during organoid initiation and in vivo following tissue damage. TET1-mediated hydroxymethylation licences differentiated ductal cells to initiate organoids and activate the regenerative programme through the transcriptional regulation of stem-cell genes and regenerative pathways including the YAP-Hippo signalling.In Europe, Trichomonas gallinae recently emerged as a cause of epidemic disease in songbirds. A clonal strain of the parasite, first found in the United Kingdom, has become the predominant strain there and spread to continental Europe. Discriminating this epidemic strain of T. gallinae from other strains necessitated development of multilocus sequence typing (MLST). This enabled construction of a robust 19 locus MLST based on existing typing loci for Trichomonas vaginalis and T. gallinae. Our MLST has the sensitivity to discriminate strains within existing genotypes confidently, and resolves the American finch A1 genotype from the European finch epidemic A1 genotype.